Abstract
Abstract
Background
Previous studies have shown that changes in intestinal microfloras are associated with both gastrointestinal (GI) and non-GI tumors. It is not clear whether there is an association between GI microflora changes and hematological malignancies.
Methods
In the current study, we used 16S rDNA gene sequencing techniques to profile the GI microbiome in children with lymphoblastic leukemia (ALL, n = 18) and matched healthy control (n = 18). Using multiple specialized software [Heatmap, Principal coordinates analysis (PCoA), Claster and Metastates], we analyzed the sequencing data for microfloral species classification, abundance and diversity.
Results
A total of 27 genera between the ALL and control groups (FDR ≤ 0.05 and/or P ≤ 0.05) showed significantly different abundance between ALL patients and healthy controls: 12 of them were predominant in healthy group and other 15 species were significantly higher in ALL group. In addition, we compared the abundance and diversity of microfloral species in ALL patients prior to and during remission stage after chemotherapy, and no significant difference was detected.
Conclusions
Compared to healthy controls, ALL patient showed significant changes of GI microfloras. Further explorations of the intestinal micro-ecology in ALL patients may provide important information to understand relationship between microfloras and ALL.
Publisher
Springer Science and Business Media LLC
Subject
Pediatrics, Perinatology, and Child Health
Reference24 articles.
1. Kaatsch P. Epidemiology of children cancer. Cancer Treat Rev. 2010;36(4):277–85.
2. Hashemizadeh H, Boroumand H, Noori R, Darabian M. Socioeconomic status and other characteristics in childhood leukemia. Iran J Ped Hematol Oncol. 2013;3(1):182–6.
3. Tong N, Xu B, Shi D, Du M, Li X, Sheng XJ, et al. Hsa-miR-196a2 polymorphism increases the risk of acute lymphoblastic leukemia in Chinese children. Mutat Res. 2014;759:16–21.
4. SHAQ Multicenter Study Group of Children’s Acute Lymphoblastic Leukemia Research. Multi-center trial based on SCMC-ALL-2005 for children’s acute lymphoblastic leukemia. Zhonghua Er Ke Za Zhi. 2013;51(7):495–501.
5. Zhao L, Liu X, Wang C, Yan KK, Lin XJ, Li S, et al. Magnetic fields exposure and childhood leukemia risk: a meta-analysis based on 11,699 cases and 13,194 controls. Leuk Res. 2014;38(3):269–74.
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献