Author:
Zhang Xiaoai,Hai Rong,Wei Jianchun,Cui Zhigang,Zhang Enmin,Song Zhizhong,Yu Dongzheng
Abstract
Abstract
Background
Yersinia pestis, the aetiological agent of plague, has been well defined genotypically on local and worldwide scales. In November 2005, five cases of severe pneumonia of unknown causes, resulting in two deaths, were reported in Yulong, Yunnan province. In this study, we compared Y. pestis isolated from the Yulong focus to strains from other areas.
Results
Two hundred and thirteen Y. pestis strains collected from different plague foci in China and a live attenuated vaccine strain of Y. pestis (EV76) were genotyped using multiple-locus variable-number tandem repeat analysis (MLVA) on 14 loci. A total of 214 Y. pestis strains were divided into 85 MLVA types, and Nei's genetic diversity indices of the various loci ranged between 0.02 - 0.76. Minimum spanning tree analysis showed that Y. pestis in China could be divided into six complexes. It was observed that Microtus strains were different from the other three biovar strains. Each plague focus had its own unique MLVA types.
Conclusion
The strains isolated from Yulong, Yunnan province had a unique MLVA type, indicating a new clone group. Our results suggest that Yulong strains may have a close relationship with strains from the Qinghai-Tibet Plateau plague focus.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference25 articles.
1. Perry RD, Fetherston JD: Yersinia pestis-etiologic agent of plague. Clin Microbiol Rev. 1997, 10: 35-66.
2. Anonymous: Human plague in 1992. Wkly Epidemiol Rec. 1994, 69: 8-10.
3. Ratsitorahina M, Chanteau S, Rahalison L, Ratsifasoamanana L, Boisier P: Epidemiological and diagnostic aspects of the outbreak of pneumonic plague in Madagascar. Lancet. 2000, 355: 111-113. 10.1016/S0140-6736(99)05163-6.
4. Centers for Disease Control and Prevention (CDC): Update: human plague-India. MMWR Morb Mortal, Wkly Rep. 1994, 43: 761-762.
5. Broussard LA: Biological agents: weapons of warfare and bioterrorism. Mol Diagn. 2001, 6: 323-333.
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献