Potentilla reptans L. preconditioning regulates H19 and MIAT long noncoding RNAs in H9C2 myoblasts Ischemia/Reperfusion model-Reference-Cited by-同舟云学术

Potentilla reptans L. preconditioning regulates H19 and MIAT long noncoding RNAs in H9C2 myoblasts Ischemia/Reperfusion model

Published:2023-07-31 Issue:1 Volume:23 Page:
ISSN:2662-7671
Container-title:BMC Complementary Medicine and Therapies
language:en
Short-container-title:BMC Complement Med Ther

Author:

Mirzaei Hassan,Salehi Aref,Javan Bita,Enayati Ayesheh,nabi Morteza Olad,Zahedi Mehdi,Zengin Gokhan

Abstract

AbstractThe present study aimed to evaluate the effect of the ethyl acetate fraction of P. reptans root (PEF) preconditioning on expressions of lncRNAs H19 and MIAT in H9C2 myoblasts I/R injury.H9C2 cells were treated with different concentrations ranging from (10–400 µg/ml) of PEF for 24 h, followed by simulation of I/R condition. For I/R experiments, H9C2 cells were subjected with the oxygen and glucose deprivation for 2 h.H9C2 cell viability was significantly enhanced by PEF preconditioning under I/R condition in a concentration-dependent manner up to 200 µg/ml as a EC50. The PEF significantly diminished the expression of lncRNA MIAT and rate of apoptosis against the I/R group. In addition, PEF pretreated before stimulation I/R condition increased H19 expression compared to the normal PEF group with no statistically significant differences between groups. Hence, the results suggest that PEF can protect cardiomyocytes during hypoxia-induced myocardial cell injury by targeting specific involved genes.

Publisher

Springer Science and Business Media LLC

Subject

Complementary and alternative medicine

Link

https://link.springer.com/content/pdf/10.1186/s12906-023-04071-z.pdf

Reference30 articles.

1. Balakumar P, Singh H, Singh M, Anand-Srivastava MB. The impairment of preconditioning-mediated cardioprotection in pathological conditions. Pharmacol Res. 2009;60:18–23. https://doi.org/10.1016/j.phrs.2009.03.002.

2. Enayati A, Yassa N, Mazaheri Z, Rajaei M, Pourabouk M, Ghorghanlu S, Basiri S, Khori V. Cardioprotective and anti-apoptotic effects of Potentilla reptans L. root via Nrf2 pathway in an isolated rat heart ischemia/reperfusion model. Life Sci. 2018;215:216–26. https://doi.org/10.1016/j.lfs.2018.11.021.

3. Sabatino J, De Rosa S, Leo I, Strangio A, Spaccarotella C, Polimeni A, Sorrentino S, Di Salvo G, Indolfi C (2021) Prediction of Significant Coronary Artery Disease Through Advanced Echocardiography: Role of Non-invasive Myocardial Work. Front Cardiovasc Med 8:719603 https://doi.org/10.3389/fcvm.2021.719603.

4. Enayati A, Salehi A, Alilou M, Stuppner H, Polshekan M, Rajaei M, Pourabouk M, Jabbari A, Mazaheri Z, Yassa N, Moheimani HR, Khori V (2021) Potentilla reptans L. postconditioning protects reperfusion injury via the RISK/SAFE pathways in an isolated rat heart. BMC Complement Med Ther 21:288 https://doi.org/10.1186/s12906-021-03456-2.

5. Gao W, Zhu M, Wang H, Zhao S, Zhao D, Yang Y, Wang ZM, Wang F, Yang ZJ, Lu X, Wang LS. Association of polymorphisms in long non-coding RNA H19 with coronary artery disease risk in a Chinese population. Mutat Res. 2015;772:15–22. https://doi.org/10.1016/j.mrfmmm.2014.12.009.