Abstract
Abstract
Background
Germacrone is one of the natural bioactive compounds found in Rhizoma curcuma essential oils. In this study, the potential anti-cancer effect of germacrone in gastric cancer cell line BGC823 was investigated.
Methods
The cell viability and proliferative activity were assessed, and cell cycle analysis was also performed. Hoechst 33258 and Annexin V/PI double staining was used for detection of cell apoptosis. Protein profiles of cell cycle-related and apoptosis-related proteins were assessed.
Results
MTT assay revealed that germacrone had marked cytotoxicity on BGC823 cells. Germacrone induced cell cycle arrest in the G2/M phase via remarkably decreased expression levels of cyclin B1, cdc 2 and cdc 25c. In addition, the treatment with germacrone induced caspase-3 activity and PARP cleavage. These findings demonstrated the effects of germacrone on inhibiting cell proliferation through induction of G2/M phase cell cycle arrest and promotion of cell apoptosis. It also indicated that germacrone functioned through modulations of cell cycle-associated protein expression and mitochondria-mediated apoptosis.
Conclusion
These findings will be valuable as the molecular basis for the germacrone-mediated anti-cancer effect against gastric cancer.
Funder
Key Research and Development Project of Shandong Province
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine
Reference46 articles.
1. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J cancer Wiley Online Library. 2015;136:E359–86.
2. Siegel RL, Miller KD, Fedewa SA, Ahnen DJ, Meester RGS, Barzi A, et al. Colorectal cancer statistics, 2017. CA Cancer J Clin Wiley Online Library. 2017;67:177–93.
3. De Vries AC, Kuipers EJ. Epidemiology of premalignant gastric lesions: implications for the development of screening and surveillance strategies. Helicobacter Wiley Online Library. 2007;12:22–31.
4. Shitara K, Kadowaki S, Nishina T, Sakai D, Yoshikawa R, Piao Y, et al. Safety, pharmacokinetic, and clinical activity profiles of ramucirumab in combination with three platinum/fluoropyrimidine doublets in Japanese patients with chemotherapy-naive metastatic gastric/gastroesophageal junction cancer. Gastric Cancer Springer. 2018;21:106–13.
5. Liu Y-H, Li M-L, Hsu M-Y, Pang Y-Y, Chen I, Chen C-K, et al. Effects of a Chinese herbal medicine, Guan-Jen-Huang (Aeginetia indica Linn.), on renal cancer cell growth and metastasis. Evidence-Based Complement Altern Med. Hindawi; 2012;2012.
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