Author:
Lukubye Ben,Ajayi Clement Olusoji,Wangalwa Rapheal,Kagoro-Rugunda Grace
Abstract
Abstract
Introduction
Symphonia globulifera and Allophylus abyssinicus are used in the management of skin rashes and sores, cough, malaria, digestive diseases, stomach ache, wounds and helminthic infections among others in Uganda, Kenya, Ethiopia, Cameroon. This study aimed at determining the phytochemical profile and antimicrobial activity of these two plants.
Methods
The stem bark and leaves of both plants were collected from Bwindi Impenetrable National Park and air-dried under shade at room temperature. Cold maceration, decoction and infusion with methanol, water and ethyl acetate as solvents were used in phytochemical extraction. Preliminary qualitative screening and thin layer chromatography were used for phytochemical profiling. Antimicrobial activity was analysed by agar well diffusion assay, broth macro-dilution assay and fractional inhibition concentration index (FICI).
Results
The leaves and stem bark of both plants have a diverse set of phytochemical compounds of variable polarity including, tannins, alkaloids, flavonoids, saponins, quinones and anthraquinones among others. Generally, methanol and water extracts of S. globulifera and A. abyssinicus had in-vitro bactericidal activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa but weak fungistatic activity against Candida albicans. Allophylus abyssinicus leaf water and S. globulifera leaf methanol extract combination had a synergistic activity (ΣFICI = 0.37) against S. aureus. Similarly, A. abyssinicus stem bark water extract and A. abyssinicus leaf water extract combination had an additive effect (ΣFICI = 1) against P. aeruginosa.
Conclusion
The leaves and stem bark crude extracts of S. globulifera and A. abyssinicus possess a wide range of bioactive phytochemical compounds but have weak antimicrobial activity against S. aureus, E. coli, P. aeruginosa and C. albicans.
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine
Reference92 articles.
1. Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules. 2016;21(5):559. https://doi.org/10.3390/molecules21050559.
2. World Health Organization. WHO Global report on traditional and complementary medicine 2019. In World Health Organization. 2019 https://apps.who.int/iris/bitstream/handle/10665/312342/9789241515436-eng.pdf?ua=1
3. WHO. WHO Traditional Medicine Strategy 2002–2005. In World Health Organisation Geneva. World Health Organization. 2002. http://www.wpro.who.int/health_technology/book_who_traditional_medicine_strategy_2002_2005.pdf
4. Oyebode O, Kandala NB, Chilton PJ, Lilford RJ. Use of traditional medicine in middle-income countries: a WHO-SAGE study. Health Policy Plan. 2016;31(8):984–91. https://doi.org/10.1093/heapol/czw022 Epub 2016 Mar 30. PMID: 27033366; PMCID: PMC5013777.
5. Qi Z. The WHO traditional medicine strategy 2014–2023: Background and progress in the last decade. Global Health History Seminar on Traditional Medicine and Ayurveda. 2015. p. 1–28.