Author:
Al Kait F.,Joris Benjamin R.,Daisley Brendan A.,Chmiel John A.,Bjazevic Jennifer,Reid Gregor,Gloor Gregory B.,Denstedt John D.,Razvi Hassan,Burton Jeremy P.
Abstract
Abstract
Background
Inquiry of microbiota involvement in kidney stone disease (KSD) has largely focussed on potential oxalate handling abilities by gut bacteria and the increased association with antibiotic exposure. By systematically comparing the gut, urinary, and oral microbiota of 83 stone formers (SF) and 30 healthy controls (HC), we provide a unified assessment of the bacterial contribution to KSD.
Results
Amplicon and shotgun metagenomic sequencing approaches were consistent in identifying multi-site microbiota disturbances in SF relative to HC. Biomarker taxa, reduced taxonomic and functional diversity, functional replacement of core bioenergetic pathways with virulence-associated gene markers, and community network collapse defined SF, but differences between cohorts did not extend to oxalate metabolism.
Conclusions
We conclude that multi-site microbiota alteration is a hallmark of SF, and KSD treatment should consider microbial functional restoration and the avoidance of aberrant modulators such as poor diet and antibiotics where applicable to prevent stone recurrence.
Funder
W. Garfield Weston Foundation
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Cited by
1 articles.
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