Forensically informative nucleotide sequencing (FINS) for the authentication of Chinese medicinal materials

Author:

Li Ming,Zhang Kalin Yan-Bo,But Paul Pui-Hay,Shaw Pang-Chui

Abstract

AbstractChinese medicinal materials may be authenticated by molecular identification. As a definitive approach to molecular identification of medicinal materials, forensically informative nucleotide sequencing (FINS) comprises four steps, namely (1) DNA extraction from biological samples, (2) selection and amplification of a specific DNA fragment, (3) determination of the sequence of the amplified DNA fragment and (4) cladistic analysis of the sample DNA sequence against a DNA database. Success of the FINS identification depends on the selection of DNA region and reference species. This article describes the techniques and applications of FINS for authenticating Chinese medicinal materials.

Publisher

Springer Science and Business Media LLC

Subject

Complementary and alternative medicine,Pharmacology

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