Author:
Zhang Ming,Li Aoqiang,Yang Qifang,Li Jingyi,Zheng Lihua,Wang Guannan,Sun Ying,Huang Yanxin,Zhang Muqing,Song Zhenbo,Liu Lei
Abstract
Abstract
Background
The realization of the “microbiota–gut–brain” axis plays a critical role in neuropsychiatric disorders, particularly depression, is advancing rapidly. Matrine is a natural bioactive compound, which has been found to possess potential antidepressant effect. However, the underlying mechanisms of regulation of the “microbiota–gut–brain” axis in the treatment of depression by oral matrine remain elusive.
Methods
Its antidepressant effects were initially evaluated by behavioral tests and relative levels of monoamine neurotransmitters, and matrine has been observed to attenuate the depression-like behavior and increase neurotransmitter content in CUMS-induced mice. Subsequently, studies from the “gut” to “brain” were conducted, including detection of the composition of gut microbiota by 16S rRNA sequencing; the metabolomics detection of gut metabolites and the analysis of differential metabolic pathways; the assessment of relative levels of diamine oxidase, lipopolysaccharide, pro-inflammatory cytokines, and brain-derived neurotrophic factor (BDNF) by ELISA kits or immunofluorescence.
Results
Matrine could regulate the disturbance of gut microbiota and metabolites, restore intestinal permeability, and reduce intestinal inflammation, thereby reducing the levels of pro-inflammatory cytokines in peripheral blood circulation and brain regions, and ultimately increase the levels of BDNF in brain.
Conclusion
Matrine may ameliorate CUMS-induced depression in mice by modulating the “microbiota–gut–brain” axis.
Funder
the Research Foundation of Jilin Provincial Science & Technology Development
the Foundation of Jilin Province Development and Reform Commission
the Foundation of Human Resources and Social Security Department of Jilin Province
the Fundamental Research Funds for the Central Universities
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
3 articles.
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