The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes
-
Published:2024-01-25
Issue:1
Volume:22
Page:
-
ISSN:1741-7007
-
Container-title:BMC Biology
-
language:en
-
Short-container-title:BMC Biol
Author:
Ryazansky Sergei S., Chen Chujia, Potters Mark, Naumenko Anastasia N., Lukyanchikova Varvara, Masri Reem A., Brusentsov Ilya I., Karagodin Dmitriy A., Yurchenko Andrey A., dos Anjos Vitor L., Haba Yuki, Rose Noah H., Hoffman Jinna, Guo Rong, Menna Theresa, Kelley Melissa, Ferrill Emily, Schultz Karen E., Qi Yumin, Sharma Atashi, Deschamps Stéphane, Llaca Victor, Mao Chunhong, Murphy Terence D., Baricheva Elina M., Emrich Scott, Fritz Megan L., Benoit Joshua B., Sharakhov Igor V., McBride Carolyn S., Tu Zhijian, Sharakhova Maria V.ORCID
Abstract
Abstract
Background
Understanding genome organization and evolution is important for species involved in transmission of human diseases, such as mosquitoes. Anophelinae and Culicinae subfamilies of mosquitoes show striking differences in genome sizes, sex chromosome arrangements, behavior, and ability to transmit pathogens. However, the genomic basis of these differences is not fully understood.
Methods
In this study, we used a combination of advanced genome technologies such as Oxford Nanopore Technology sequencing, Hi-C scaffolding, Bionano, and cytogenetic mapping to develop an improved chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus.
Results
We then used this assembly to annotate odorant receptors, odorant binding proteins, and transposable elements. A genomic region containing male-specific sequences on chromosome 1 and a polymorphic inversion on chromosome 3 were identified in the Cx. quinquefasciatus genome. In addition, the genome of Cx. quinquefasciatus was compared with the genomes of other mosquitoes such as malaria vectors An. coluzzi and An. albimanus, and the vector of arboviruses Ae. aegypti. Our work confirms significant expansion of the two chemosensory gene families in Cx. quinquefasciatus, as well as a significant increase and relocation of the transposable elements in both Cx. quinquefasciatus and Ae. aegypti relative to the Anophelines. Phylogenetic analysis clarifies the divergence time between the mosquito species. Our study provides new insights into chromosomal evolution in mosquitoes and finds that the X chromosome of Anophelinae and the sex-determining chromosome 1 of Culicinae have a significantly higher rate of evolution than autosomes.
Conclusion
The improved Cx. quinquefasciatus genome assembly uncovered new details of mosquito genome evolution and has the potential to speed up the development of novel vector control strategies.
Funder
Division of Intramural Research, National Institute of Allergy and Infectious Diseases Russian Science Foundation New York Stem Cell Foundation National Institute of Food and Agriculture Tomsk State University NCBI, NLM, NIH Institute of Cytology and Genetics SBRAS, Russia
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Developmental Biology,Plant Science,General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Physiology,Ecology, Evolution, Behavior and Systematics,Structural Biology,Biotechnology
Reference155 articles.
1. WHO. Vector-borne diseases, 2020. https://www.who.int/news-room/fact-sheets/detail/vector-borne-diseases. Accessed 21Aug 2023. 2. Pfirer. Six mosuito diseases that can be deadly. 2023. Accessed 21August 2023. 3. CDC. Fighting the world’s deadliest animal. 2023. 2019. https://www.cdc.gov/globalhealth/stories/2019/world-deadliest-animal.html. Accessed 21 Aug 2023. 4. Wang GH, Du J, Chu CY, Madhav M, Hughes GL, Champer J. Symbionts and gene drive: two strategies to combat vector-borne disease. Trends Genet. 2022;38(7):708–23. 5. Berlin K, Koren S, Chin CS, Drake JP, Landolin JM, Phillippy AM. Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nat Biotechnol. 2015;33(6):623–30.
|
|