Abstract
AbstractLeishmaniasis is a vector-borne disease, one of the most important neglected tropical diseases. Existing anti-leishmanial treatments are not effective for a long time and associated with toxic side effects so searching for a new, effective and safe alternative treatments against infectious diseases is greatly needed. This study is aimed to assess the leishmaniacidal effects of methanolic extracts of Eryngium planum (E. planum) and Ecbilliun elaterum (E. elaterum) on Leishmania major (L. major), In vitro. The selected plants were collected from northern areas of Iran. The methanolic extract from the aerial parts of plants were prepared using maceration methods. GC- Mass analysis was used to determine the compounds of the plants. Promastigotes of L. major was cultured in RPMI-1640 medium and the anti-leishmanial and cytotoxicity effects of extracts at concentrations of 100, 200, 400 and 800 µg/ml were assessed using MTT assay. The data obtained from gas chromatography revealed that α-Pinene, Caryophyllene oxide, β‐Caryophyllene, Bicyclogermacrene and α‐Bisabolol are the main compounds extracted from E. planum and α‐Pinene, Germacrene D, Caryophyllene oxide, γ‐Eudesmol and α‐Bisabolol are the main components of E. elaterum. The results of MTT Assay revealed that E. planum at concentrations of 800 µg/ml after 24 h at 400 µg/ml after 48 h and the E. elaterium at concentrations of 800 µg/ml after 48 h at 400 µg/ml after 72 h had similar anti-leishmanial effects to the positive control. These results indicated that E. planum and E. elaterum are the potential sources for the discovery of novel anti-leishmanial treatments.
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,Biophysics
Reference50 articles.
1. Abed AY (2009) Cytotoxicity of Fumaria officinalis on Leishmania tropica, l20b cell lines and bacteria in vitro. J Glob Pharma Technol 11(03)
2. Adwan G, Salameh Y, Adwan K (2011) Effect of ethanolic extract of Ecballium elaterium against Staphylococcus aureus and Candida albicans. Asian Pac J Trop Biomed 1(6):456–460
3. Akbari F, Azadbakht M, Gaurav A, Azimi F, Mahdizadeh Z, Vahedi L, Barzegar Nejad A, Chabra A, Eghbali M (2022) Evaluation of the therapeutic effect of the traditional herbal medicine atrifil and oshagh gum on testosterone-induced Benign Prostatic Hyperplasia in Wistar rats. Adv Urol 2022
4. Al-Askar AA, Bashir S, Mohamed AE, Sharaf OA, Nabil R, Su Y, Abdelkhalek A, Behiry SI (2023) Antimicrobial efficacy and hplc analysis of polyphenolic compounds in a whole-plant extract of eryngium campestre. Separations 10(6):362
5. Al-Rubaye AF, Hameed IH, Kadhim MJ (2017) A review: uses of gas chromatography-mass spectrometry (gc-ms) technique for analysis of bioactive natural compounds of some plants. Int J Toxicol Pharmacol Res 9(1):81–85