Author:
Geiger Martin,Oppi Sara,Nusser-Stein Stefanie,Costantino Sarah,Mohammed Shafeeq Ahmed,Gorica Era,Hoogerland Joanne A.,Matter Christian M.,Guillaumon Ana T.,Ruschitzka Frank,Paneni Francesco,Oosterveer Maaike H.,Stein Sokrates
Abstract
Abstract
Background
The nuclear receptor corepressor 1 (NCOR1) plays an important role in the regulation of gene expression in immunometabolic conditions by connecting chromatin-modifying enzymes, coregulators and transcription factors. NCOR1 has been shown to be involved in cardiometabolic diseases. Recently, we demonstrated that the deletion of macrophage NCOR1 aggravates atherosclerosis by promoting CD36-triggered foam cell formation via PPARG derepression.
Purpose
Since NCOR1 modulates the function of several key regulators involved in hepatic lipid and bile acid metabolism, we hypothesized that its deletion in hepatocytes alters lipid metabolism and atherogenesis.
Methods
To test this hypothesis, we generated hepatocyte-specific Ncor1 knockout mice on a Ldlr-/- background. Besides assessing the progression of the disease in thoracoabdominal aortae en face, we analyzed hepatic cholesterol and bile acid metabolism at expression and functional levels.
Results
Our data demonstrate that liver-specific Ncor1 knockout mice on an atherosclerosis-prone background develop less atherosclerotic lesions than controls. Interestingly, under chow diet, plasma cholesterol levels of liver-specific Ncor1 knockout mice were slightly higher compared to control, but strongly reduced compared to control mice after feeding them an atherogenic diet for 12 weeks. Moreover, the hepatic cholesterol content was decreased in liver-specific Ncor1 knockout compared to control mice. Our mechanistic data revealed that NCOR1 reprograms the synthesis of bile acids towards the alternative pathway, which in turn reduce bile hydrophobicity and enhances fecal cholesterol excretion.
Conclusions
Our data suggest that hepatic Ncor1 deletion in mice decreases atherosclerosis development by reprograming bile acid metabolism and enhancing fecal cholesterol excretion.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Rosalind Franklin Fellowship from the University of Groningen
Novartis Stiftung für Medizinisch-Biologische Forschung
Olga Mayenfisch Stiftung
Publisher
Springer Science and Business Media LLC
Subject
Cardiology and Cardiovascular Medicine,Endocrinology, Diabetes and Metabolism
Cited by
4 articles.
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