Author:
Ren Zhen-Hu,Wu Kun,Yang Rong,Liu Zhe-Qi,Cao Wei
Abstract
Abstract
Background
Our study aimed to reveal the regulatory mechanisms of miRNAs and matrix metalloproteinases (MMPs) in oral squamous cell carcinoma (OSCC).
Methods
The mRNA and miRNA expression profiles of six metastatic tumour samples, six nonmetastatic tumour samples, and six normal tissue samples were used for microarray analysis. Moreover, the important genes and miRNAs were validated by published profiles in Oncomine and by qRT-PCR.
Results
MMP7, MMP13, and MMP10 were upregulated, and MMP12 and MMP9 were downregulated in metastatic tumours compared with nonmetastatic tumours. MMP7 was regulated by miR-4697-5p and miR-7109-5p. MMP7 and MMP13 were upregulated in OSCC samples compared with normal samples in Oncomine. Moreover, qRT-PCR revealed that the expression of miR-7109-5p and miR-34b was decreased in metastatic tumours compared with nonmetastatic tumours.
Conclusions
Our study suggested that miR-7109-5p and miR-34b might play important roles in the metastasis of OSCC by regulating MMP7 and MMP13 expression, respectively.
Funder
SHIPM-mu fund
The Interdisciplinary Program of Shanghai Jiao Tong University
the Shanghai Anticancer Association EYAS PROJECT
The National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
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