Author:
Maidana Silvina S,Lomonaco Patricia M,Combessies Gustavo,Craig María I,Diodati Julian,Rodriguez Daniela,Parreño Viviana,Zabal Osvaldo,Konrad José L,Crudelli Gustavo,Mauroy Axel,Thiry Etienne,Romera Sonia A
Abstract
Abstract
Background
Parainfluenza virus type 3 (PIV3) was isolated from dairy buffaloes (Bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions.
Results
Examination of nasal and vaginal swabs collected from 12 diseased buffaloes led to the isolation of three paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).
Conclusions
This is the first characterization of BPIV3 in water buffalo.
According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C were found in cattle.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine
Reference30 articles.
1. Wang LF, Collins PL, Fouchier RAM, Kurath G, Lamb RA, Randall RE, Rima BK: family Paramyxoviridae. Virus taxonomy: Ninth report of the international committee on taxonomy of viruses. Edited by: King AMQ, Lefkowitz E, Adams MJ. Elsevier Academic Press, San Diego, California; 2011:672-685.
2. Turner RB, Couch RB: Rhinoviruses : Parainfluenza Viruses : the viruses and their replication. Fields Virology. 5th edition. Edited by: Knipe DM, Howley PM. Lippincott, Williams & Wilkins, Philadelphia; 2007:1449-1496.
3. Pringle CR, Filipiuk AH, Robinson BS, Watt PJ, Higgins P, Tyrrell DA: Immunogenicity and pathogenicity of a triple temperature-sensitive modified respiratory syncytial virus in adult volunteers. Vaccine. 1993, 11 (4): 473-478. 10.1016/0264-410X(93)90290-E.
4. Haanes EJ, Guimond P, Wardley R: The bovine parainfluenza virus type-3 (BPIV-3) hemagglutinin/neuraminidase glycoprotein expressed in baculovirus protects calves against experimental BPIV-3 challenge. Vaccine. 1997, 15 (6–7): 730-738.
5. Snowder GD, Van Vleck LD, Cundiff LV, Bennett GL: Bovine respiratory disease in feedlot cattle: environmental, genetic, and economic factors. J Anim Sci. 2006, 84 (8): 1999-2008. 10.2527/jas.2006-046.
Cited by
45 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献