Author:
Aisha Abdalrahim FA,Majid Amin Malik Shah Abdul,Ismail Zhari
Abstract
Abstract
Background
O. stamineus is a medicinal herb with remarkable pharmacological properties. However, poor solubility of the active principles limits its medicinal value. This study sought to prepare nano liposomes of OS ethanolic extract in unpurified soybean phospholipids in order to improve its solubility and permeability. OS liposomes were prepared by the conventional film method, and were characterized for solubility, entrapment efficiency, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), particle size and zeta potential, release, absorption in everted rat intestinal sacs, and DPPH scavenging effect.
Results
OS liposomes showed substantial enhancement of extract’s solubility from 956 ± 34 to 3979 ± 139 μg/ml, with entrapment efficiency of 66.2 ± 0.9%. FTIR study indicates interaction between soybean phospholipids and OS extract. TEM and dynamic light scattering showed presence of round anionic nano liposomes with particle size and zeta potential of 152.5 ± 1.1 nm and −49.8 ± 1.0 mV, respectively. A study using the fluorescent probe pyrene showed the critical micellar concentration is 9.2 ± 2.9 μg/ml. Release studies showed 94 ± 0.1% release in non-formulated extract and 62.4 ± 0.1% in OS liposomes. Released extract from OS liposomes showed improvement in DPPH scavenging effect, IC50 = 23.5 ± 1.1 μg/ml compared to 32.4 ± 0.5 μg/ml in non-formulated extract. OS liposomes were stable at pH 5.5 and 7.4, but showed reversible agglomeration at pH 1.6. Absorption in everted rat intestinal sacs showed substantial improvement in permeability of 3′-hydroxy-5, 6, 7, 4″-tetramethoxyflavone, sinensetin, eupatorin, and 3 other unknown compounds.
Conclusions
Enhanced solubility, absorption and antioxidant effect may improve the overall pharmacological effects and medicinal value of OS ethanolic extract.
Publisher
Springer Science and Business Media LLC
Reference33 articles.
1. Ahamed MBK, Aisha AFA, Nassar ZD, Siddiqui JM, Ismail Z, Omari S, Parish C, Majid AMSA: Cat’s whiskers tea (Orthosiphon stamineus) extract inhibits growth of colon tumor in nude mice and angiogenesis in endothelial cells via suppressing VEGFR phosphorylation. Nutr Cancer. 2012, 64: 89-99. 10.1080/01635581.2012.630160.
2. Arafat OM, Tham SY, Sadikun A, Zhari I, Haughton PJ, Asmawi MZ: Studies on diuretic and hypouricemic effects of orthosiphon stamineus methanol extracts in rats. J Ethnopharmacol. 2008, 118: 354-360. 10.1016/j.jep.2008.04.015.
3. Alshawsh MA, Abdulla MA, Ismail S, Amin ZA: Hepatoprotective effects of orthosiphon stamineus extract on thioacetamide-induced liver cirrhosis in rats. Evid Based Complement Altern Med. 2011, 2011: Article ID 103039, 6 pages http://dx.doi.org/10.1155/2011/103039
4. Mohamed EAH, Mohamed AJ, Asmawi MZ, Sadikun A, Ebrika OS, Yam MF: Antihyperglycemic effect of orthosiphon stamineus benth leaves extract and its bioassay-guided fractions. Molecules. 2011, 16: 3787-3801. 10.3390/molecules16053787.
5. Ho C-H, Noryati I, Sulaiman S-F, Rosma A: In vitro antibacterial and antioxidant activities of orthosiphon stamineus benth. Extracts against food-borne bacteria. Food Chem. 2010, 122: 1168-1172. 10.1016/j.foodchem.2010.03.110.
Cited by
74 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献