Morphological assessment of the dynamics of changes in the wound process when using Blastomunil and Decametoxin in an experiment

Abstract

Annotation. The purpose of the study: to carry out a morphological assessment of the effectiveness of the use of the immunomodulator Blastomunil in combination with Decamethoxine in the treatment of infected wounds in an experiment. The topicality of the topic is determined by the search for new medicinal forms for the treatment of infected wounds. At the same time, morphological assessment of the course of the wound process is extremely important. A study was conducted on 40 rats (4 groups of animals, 10 in each). An infected wound was made on the back of all animals under anesthesia. 1 group – was not treated. The 2nd group of animals was treated by local application of 0.02% Decamethoxine with application of a sterile gauze bandage; the 3rd group of animals was treated by local application of 0.075 mg of Blastomunil in 1 ml of 0.02% Decamethoxine solution and also by applying a sterile gauze bandage; the 4th group of animals was treated by a single intramuscular injection of 0.12 mg of Blastomunil at the beginning of the experiment, followed by daily local treatment of a combination of 0.075 mg of Blastomunil in 1 ml of 0.02% Decamethoxine solution and application of a sterile gauze bandage. To evaluate the morphological changes of the wound process, the animals were taken out of the experiment under anesthesia on the 3rd, 7th, 10th, and 14th day. The results. the use of a combined mixture of 0.02% Decamethoxine with Blastomunil made it possible to use the positive immunomodulatory effect of Blastomunil and the antimicrobial properties of Decamethoxine, which generally gave a better result due to the rapid cleansing of the wound, which began to cleanse already on the 3rd day and was completely cleansed on the 7th day. During treatment, “lymphocyte and macrophage saturation” of regenerative tissues was observed. Treatment with a combined mixture of 0.02% Decamethoxine and Blastomunil against the background of additional intramuscular injection of Blastomunil in animals of the 4th group did not improve (and even slightly worsened) the results of the treatment of the animals of the 3rd group. Morphological data show that the local application for therapeutic purposes of a combined mixture of 0.02% Decamethoxine with Blastomunil is appropriate and effective, due to the antimicrobial, immunomodulatory and regenerative properties of this mixture.