Voltage-dependent gating of gap junction channels in embryonic chick ventricular cell pairs

Abstract

The dependence of macroscopic gap junctional conductance (Gj) on transjunctional voltage (Vj) was studied in paired myocytes after enzymatic dissociation of 7-day-old embryonic chick ventricles. The membrane voltage of both cells was independently controlled by separate patch-clamp circuits in the whole cell configuration. Two distinctive unitary junctional conductances were identified in recordings from seven different cell pairs. The larger channel had a mean conductance of 166 +/- 37 pS (n = 6 pairs), whereas a second channel averaged 58 +/- 10 pS (n = 3). Instantaneous Gj remained linear over a Vj range of -100 to +100 mV, whereas the steady-state Gj declined when voltages exceeded +/- 30 mV. Both decay and recovery phases of Gj follow exponential time courses, with the recovery time constant being four times slower than inactivation, requiring 1.1 s at 80 mV. The normalized steady-state Gj-Vj curve could be defined by a two-state Boltzmann distribution, assuming an effective gating charge of 1.72, a half-inactivation voltage of 45 mV, and a residual voltage-insensitive Gj of 27% of maximum. Single-channel recordings revealed closure of 160-pS channels on a Vj step to 80 mV, and the ensemble average of five such records produced an exponentially decaying junctional current with a time constant of 184 ms. The single-channel current-voltage relationship remains linear with a slope of 145 pS over the entire Vj range. The results support the hypothesis that a population of 160-pS gap junction channels is gated by transjunctional potentials.