Bacterial lipopolysaccharide inhibits colonic carrier-mediated uptake of thiamin pyrophosphate: roles for TLR4 receptor and NF-κB/P38/JNK signaling pathway-Reference-Cited by-同舟云学术

Bacterial lipopolysaccharide inhibits colonic carrier-mediated uptake of thiamin pyrophosphate: roles for TLR4 receptor and NF-κB/P38/JNK signaling pathway

Published:2023-09-01 Issue:3 Volume:325 Page:C758-C769
ISSN:0363-6143
Container-title:American Journal of Physiology-Cell Physiology
language:en
Short-container-title:American Journal of Physiology-Cell Physiology

Author:

Anthonymuthu Selvaraj¹^ORCID,Sabui Subrata¹²,Lee Katherine¹,Sheikh Alaullah³,Fleckenstein James M.³⁴,Said Hamid M.¹⁵²^ORCID

Affiliation:

1. Department of Physiology & Biophysics, School of Medicine, University of California, Irvine, California, United States

2. Department of Medical Research, Tibor Rubin VA Medical Center, Long Beach, California, United States

3. Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States

4. Veterans Affairs Medical Center, St. Louis, Missouri, United States

5. Department of Medicine, School of Medicine, University of California, Irvine, California, United States

Abstract

This study examined the effect of the bacterial lipopolysaccharide (LPS) on the colonic uptake of thiamin pyrophosphate (TPP), the biologically active form of vitamin B1. Three complementary models were used: in vitro (human NCM460 cells), ex vivo (human colonoids), and in vivo (mice). The results showed LPS to significantly suppress TPP uptake and the expression of its transporter, and that these effects are mediated via the membrane TLR4 receptor, and involve the NF-κB/p38/JNK signaling pathways.

Funder

HHS | National Institutes of Health

U.S. Department of Veterans Affairs

Washington University in St. Louis

Publisher

American Physiological Society

Subject

Cell Biology,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpcell.00272.2023

Reference56 articles.

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