Human Cx50 Isoleucine177 prevents heterotypic docking and formation of functional gap junction channels with Cx43-Reference-Cited by-同舟云学术

Human Cx50 Isoleucine177 prevents heterotypic docking and formation of functional gap junction channels with Cx43

Published:2024-02-01 Issue:2 Volume:326 Page:C414-C428
ISSN:0363-6143
Container-title:American Journal of Physiology-Cell Physiology
language:en
Short-container-title:American Journal of Physiology-Cell Physiology

Author:

Wong Robert S.¹,Chen Honghong¹,Li Yi X.¹,Esseltine Jessica L.²,Stathopulos Peter B.¹,Bai Donglin¹^ORCID

Affiliation:

1. Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada

2. Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John’s, Newfoundland and Labrador, Canada

Abstract

The human lens is an avascular organ, and its transparency is partially dependent on gap junction (GJ) network composed of Cx46, Cx50, and Cx43. We found that human Cx46 can dock and form functional heterotypic GJs with Cx50 and Cx43, but Cx50 is unable to form functional heterotypic GJs with Cx43. Through mutagenesis and patch-clamp study of several designed variants, we found that Cx50 I177L was sufficient to form functional heterotypic GJs with Cx43.

Funder

Gouvernement du Canada | Canadian Institutes of Health Research

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada

Publisher

American Physiological Society

Subject

Cell Biology,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpcell.00634.2023

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