Alterations in gene expression and microbiome composition upon calcium-sensing receptor deletion in the mouse esophagus-Reference-Cited by-同舟云学术

Alterations in gene expression and microbiome composition upon calcium-sensing receptor deletion in the mouse esophagus

Published:2024-04-01 Issue:4 Volume:326 Page:G438-G459
ISSN:0193-1857
Container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology
language:en
Short-container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology

Author:

Abdulnour-Nakhoul Solange M.¹²^ORCID,Kolls Jay K.¹³^ORCID,Flemington Erik K.⁴,Ungerleider Nathan A.⁴,Nakhoul Hani N.⁴,Song Kejing¹³^ORCID,Nakhoul Nazih L.¹²^ORCID

Affiliation:

1. Deming Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana, United States

2. Department of Physiology, Tulane University School of Medicine, New Orleans, Louisiana, United States

3. Center for Translational Research in Infection and Inflammation, Tulane University School of Medicine, New Orleans, Louisiana, United States

4. Department of Pathology, Tulane University, New Orleans, Louisiana, United States

Abstract

Calcium-sensing receptor (CaSR) plays a significant role in maintaining the barrier function of esophageal epithelium. Using RNA sequencing, we show that conditional deletion of CaSR from mouse esophagus causes upregulation of genes involved in DNA replication and cell cycle and downregulation of genes involved in the innate immune response, protein translation, and cellular protein synthesis. Pathway analysis shows disruption of signaling pathways of calcium and actin cytoskeleton. These changes caused inflammation and esophageal dysbiosis.

Funder

Merit Grant VA administration

Tulane Bridge Fund

Paul Teschan Research

HHS | NIH | National Cancer Institute

HHS | NIH | National Heart, Lung, and Blood Institute

HHS | NIH | National Institute of General Medical Sciences

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpgi.00066.2023

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