Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid β-oxidation at cellular-level resolution in mice-Reference-Cited by-全球学者库

Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid β-oxidation at cellular-level resolution in mice

Published:2023-11-01 Issue:5 Volume:325 Page:E552-E561
ISSN:0193-1849
Container-title:American Journal of Physiology-Endocrinology and Metabolism
language:en
Short-container-title:American Journal of Physiology-Endocrinology and Metabolism

Author:

Matsumoto Ayumi¹^ORCID,Matsui Isao¹²^ORCID,Uchinomiya Shohei³,Katsuma Yusuke¹,Yasuda Seiichi¹,Okushima Hiroki¹,Imai Atsuhiro¹,Yamamoto Takeshi¹,Ojida Akio³,Inoue Kazunori¹,Isaka Yoshitaka¹^ORCID

Affiliation:

1. Department of Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan

2. Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan

3. Medical Chemistry and Chemical Biology, Department of Medicinal Sciences, Graduate School of Pharmaceutical Science, Kyushu University, Fukuoka, Japan

Abstract

Fatty acid β-oxidation (FAO) plays a key role in energy homeostasis. Here, the authors established a strategy for visualizing FAO activity in vivo at the cellular-level spatial resolution and seconds-level time resolution in mice. Quantitative analysis revealed spatiotemporal heterogeneity in hepatic FAO dynamics. Our method is widely applicable because it is simple and uses a multiphoton microscope to observe the FAOBlue-injected mice.

Funder

Kidney Foundation, Japan

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpendo.00147.2023

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