Resveratrol ameliorates metabolic disorders and muscle wasting in streptozotocin-induced diabetic rats


Chen Kuan-Hsing12,Cheng Mei-Ling3,Jing Yu-Hong4,Chiu Daniel Tsun-Yee3,Shiao Ming-Shi5,Chen Jan-Kan6


1. Graduate Institute of Clinical Medicine, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan;

2. Kidney Research Center, Department of Nephrology, Chang Gung Memorial Hospital, Lin-Kou Medical Center

3. Graduate Institute of Medical Biotechnology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan;

4. Institute of Anatomy and Embryology, School of Basic Medical Sciences, LanZhou University, LanZhou, China;

5. Department of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan;

6. Department of Physiology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; and


Diabetes mellitus (DM) is characterized by dysregulated energy metabolism. Resveratrol (RSV) has been shown to ameliorate hyperglycemia and hyperlipidemia in diabetic animals. However, its overall in vivo effects on energy metabolism and the underlying mechanism require further investigation. In the present study, electrospray ionization-tandem mass spectrometry was employed to characterize the urine and plasma metabolomes of control, streptozotocin-induced DM and RSV-treated DM rats. Using principal component analysis (PCA) and heat map analysis, we discovered significant differences among control and experimental groups. RSV treatment significantly reduced the metabolic abnormalities in DM rats. Compared with the age-matched control rats, the level of carnitine was lower, and the levels of acetylcarnitine and butyrylcarnitine were higher in the urine and plasma of DM rats. RSV treatment ameliorated the deranged carnitine metabolism in DM rats. In addition, RSV treatment attenuated the diabetic ketoacidosis and muscle protein degradation, as evidenced from the attenuation of elevated urinary methyl-histidine and plasma branched-chain amino acids levels in DM rats. The beneficial effects of RSV in DM rats were correlated with activation of hepatic AMP-activated protein kinase and SIRT1 expression, increase of hepatic and muscular mitochondrial biogenesis and inhibition of muscle NF-κB activities. We concluded that RSV possesses multiple beneficial metabolic effects in insulin-deficient DM rats, particularly in improving energy metabolism and reducing protein wasting.


American Physiological Society


Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism







Copyright © 2019-2023 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3