Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing-Reference-Cited by-同舟云学术

Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing

Published:2024-05-01 Issue:5 Volume:326 Page:E723-E734
ISSN:0193-1849
Container-title:American Journal of Physiology-Endocrinology and Metabolism
language:en
Short-container-title:American Journal of Physiology-Endocrinology and Metabolism

Author:

Yang Huan¹,Luo Junming²,Liu Xuyang³,Luo Yue³,Lai Xiaoyang³,Zou Fang³^ORCID

Affiliation:

1. Department of Endocrinology, Jiujiang University Affiliated Hospital, Jiujiang, People’s Republic of China

2. Department of Respiratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China

3. Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China

Abstract

In this study, we created a comprehensive single-cell atlas of pancreatic islets in a T1D mouse model using scRNA-Seq and identified 11 major cell types in the islets, highlighting the role of alpha and beta cells in T1D. This study revealed a significant reduction in the maturity alpha and beta cells in T1D mice through flow cytometry. It also demonstrated the heterogeneity of alpha and beta cells, potentially crucial for T1D progression. Overall, our scRNA-Seq analysis provided new insights for understanding and treating T1D by studying cell subtype changes and functions.

Funder

Jiangxi Education Department Grant

Jiangxi Provincial Health Commission Grant

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpendo.00323.2023

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