Green tea epigallocatechin gallate enhances cardiac function restoration through survival signaling expression in diabetes mellitus rats with autologous adipose tissue-derived stem cells

Author:

Chen Tung-Sheng12,Liou Show-Yih3,Kuo Chia-Hua4,Pan Lung-Fa56,Yeh Yu-Lan78,Liou Jeffery9,Padma V. Vijaya10,Yao Chun-Hsu1111213,Kuo Wei-Wen14,Huang Chih-Yang2151617

Affiliation:

1. Biomaterials Translational Research Center, China Medical University Hospital, Taichung, Taiwan;

2. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan;

3. Formosan Blood Purification Foundation, Taipei, Taiwan;

4. Department of Sports Sciences, University of Taipei, Taipei, Taiwan;

5. Division of Cardiology, Armed Force Taichung General Hospital, Taichung, Taiwan;

6. Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung, Taiwan;

7. Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan;

8. Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan;

9. Comprehensive Weight Management Center, Taipei Medical University Hospital, Taipei, Taiwan;

10. Department of Biotechnology, Bharathiar University, Coimbatore, India;

11. Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan;

12. School of Chinese Medicine, China Medical University, Taichung, Taiwan;

13. Department of Biomedical Informatics, Asia University, Taichung, Taiwan;

14. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan;

15. Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan;

16. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan; and

17. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam

Abstract

The present study tests a hypothesis that cardioprotective effects mediated by autologous adipose-derived stem cells (ADSC) in rats afflicted with insulin-dependent diabetes mellitus (IDDM) may be synergistically enhanced by oral treatment with green tea epigallocatechin gallate (EGCG). Wistar rats were divided into sham, DM, DM+ADSC (autologous transplanted 1 × 106 cells per rat), and DM+ADSC+E (E, green tea oral administration EGCG). Heart tissues were isolated from all rats, and investigations were performed after 2-mo treatment. In the sham, DM, and DM+ADSC groups, we found that DM induced cardiac dysfunction (sham and DM) and autologous ADSC transplantation could partially recover cardiac functions (DM and DM+ADSC) in DM rats. Compared with DM+ADSC, significant improvement in cardiac functions can be observed in DM+ADSC+E in echocardiographic data, histological observations, and even cellular protein expression. Oral green tea EGCG administration and autologous ADSC transplantation show synergistically beneficial effects on diabetic cardiac myopathy in DM rats. NEW & NOTEWORTHY Cardiomyopathy can be induced in rats with diabetes mellitus (DM). Heart function can be restored in DM rats with adipose-derived stem cell treatment. Oral epigallocatechin gallate (EGCG) administration synergistically enhances cardiac function in DM rats with stem cell treatment. The EGCG and stem cell treatment cross-effect occurs via survival protein expression.

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology

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