Author:
Yi Kyung,Lee Hong,Lee Sang-Rae,Lee Youngjeon,Lee Seung,Lee Chulhyun,Cha Sang-Hoon
Abstract
AbstractPrevious reports revealed that middle cerebral artery occlusion (MCAO) models in rats were very diverse in nature, and experimental stroke of a more homogenous nature had not been previously documented. This paper aims to present our novel observations of experimental stroke in rats with similar MRI characteristics after MCAO. Immediately after MCAO, 19 rats were placed into a 4.7 T MRI scanner, and diffusion weighted imaging (DWI) of axial and coronal planes was repeated every 10 minutes up to post-occlusion 115 minutes. Apparent diffusion coefficient (ADC) values of the ischemic lesions were calculated and compared to those of the unaffected contra-lateral hemispheres. Successful MCAO was defined when the whole left MCA territory showed ADC abnormality on DWI. Percentage of hemispheric lesion volume (% HLV), relative ADC value (rADC), and relative DWI signal intensity (rDWI) were serially evaluated for quantitative analysis of ADC-derived lesion characteristics. Successful MCA territorial infarction was induced in nine rats (9/19, 47.4%). In quantitative analysis of ADC-derived lesion characteristics, lesion volumes of seven rats (group 1) were very similar, but larger than those of the other two rats (group 2): % HLV of initial MRI = 45.4 ± 2.5 / 19.1 ± 6.6. rADCs and rDWIs of group 1 showed similar patterns of temporal change, which was different from those of group 2. Using prospective diffusion MRI after MCAO in rats, we identified territorial hyperacute ischemic lesions with similar MRI characteristics. This observation would contribute to the establishment of more homogenous rodent models for ischemic stroke translational research.
Reference37 articles.
1. Kilkenny C., Browne W.J., Cuthill I.C., Emerson M., Altman D.G., Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research, J. Pharmacol.Pharmacother., 2010, 1, 94–99
2. Donnan G.A., Fisher M., Macleod M., Davis S.M., Stroke, Lancet, 2008, 371, 1612–1623
3. Philip M., Benatar M., Fisher M., Savitz S.I., Methodological quality of animal studies of neuroprotective agents currently in phase II/III acute ischemic stroke trials, Stroke, 2009, 40, 577–581
4. Koizumi JY.Y., Nakazawa T, Ooneda G., Experimental studies of ischemic brain edema, I: a new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area, Jpn. J. Stroke, 1986, 1–8
5. Macrae I.M., Preclinical stroke research — advantages and disadvantages of the most common rodent models of focal ischaemia, Br. J. Pharmacol., 2011, 164, 1062–1078
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献