Affiliation:
1. 1Masaryk University
2. 2Tomas Bata University in Zlín
Abstract
AbstractPresented work summarizes the data about polyphenolic profiles in various plant parts (leaves, shoots, berries) of sea buckthorn (Hippophaë rhamnoides L.) during the annual growth cycle. A reversed-phase high performance liquid chromatography method (RP-HPLC) coupled with diode-array detection (DAD) was optimized for determination of catechin, epicatechin, gallic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin (quercetin 3-rutinoside) and quercitrin (quercetin 3-rhamnoside). The content of these polyphenolic compounds was monitored in extracts of sea buckthorn plant samples from April to October. The total antioxidant activity was determined using scavenging of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonate) cation radical (ABTS·+) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·). The total content of polyphenols was estimated by conventional spectrophotometric method using Folin-Ciocalteu reagent. The monitoring of temporal changes of selected polyphenolic compounds by RP-HPLC showed that catechin, epicatechin and gallic acid were the most abundant analytes in annual green shoots and leaves, and their content varied significantly during the studied period.
Subject
Materials Chemistry,General Chemistry
Reference41 articles.
1. P. Valíček, E.V. Havelka, Sea buckthorn — a plant for future (START Publ., Benešov, 2008) (in Czech)
2. T.S.C. Li, W.R. Schroeder, Horttechnology 6, 370 (1996)
3. G. Suryakumar, A. Gupta, J. Ethnopharmacol. 138, 268 (2011)
4. P.E. Miller, D.C. Snyder, Nutrition in Clinical Practise 27, 599 (2012)
5. R. Tsao, Nutrients 2, 1231 (2010)