Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China
Author:
Hu Wen1, Xu Xin1, Liu Qiang1, Ji Jun1, Kan Yunchao1, Yao Lunguang1, Bi Yingzuo2, Xie Qingmei2
Affiliation:
1. Henan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Nanyang Normal University , Nanyang , , PR China 2. College of Animal Science, South China Agricultural University , Guangzhou , , PR China
Abstract
Abstract
Introduction
Canine parvovirus (CPV) disease is one of the most threatening to domestic and wild dogs.
Material and Methods
A total of 132 clinical samples were isolated from domestic dogs with diarrhoea from Henan, Hubei, Jiangsu, and Anhui provinces from 2016 to 2017, and 56 were positive for CPV-2 by PCR. A phylogenetic tree was constructed for the isolate sequences incorporating 53 non-Chinese reference strains.
Results
VP2 sequences showed the strains mainly to be new CPV-2a/2b and CPV-2c genotypes. The Ala5Gly, Phe267Tyr, Ser297Ala, Tyr324Ile, Gln370Arg, Asn426Asp or Asn426Glu, and Thr440Ala sites in the VP2 protein antigenic region were found to have high mutation rates. The VP2 tertiary structural model shows that the change at these mutation points is a factor for the changes in the protein structure. Significant differences between the Central Chinese strains and others were found, indicating that evolution is geographically related and extended in major regions. The homology between the identified strains confirmed their relationship. Phylogenetic analysis indicated that the common genotypes in the same clusters differ slightly in homology and evolutionary history.
Conclusion
This epidemiological study enriches the available data and serves as an important reference for studies on the evolution of CPV and selection of vaccines in China.
Publisher
Walter de Gruyter GmbH
Subject
General Veterinary
Reference20 articles.
1. Battilani M., Balboni A., Ustulin M., Giunti M., Scagliarini A., Prosperi S.: Genetic complexity and multiple infections with more Parvovirus species in naturally infected cats. Vet Res 2011, 42, 43. 2. Cotmore S.F., Agbandje-McKenna M., Chiorini J.A., Mukha D.V., Pintel D.J., Qiu J., Soderlund-Venermo M., Tattersall P., Tijssen P., Gatherer D., Davison A.J.: The family Parvoviridae. Arch Virol 2014, 159, 1239–1247. 3. Decaro N., Desario C., Campolo M., Elia G., Martella V., Ricci D., Lorusso E., Buonavoglia C.: Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant. J Vet Diagn Invest 2005, 17, 133–138. 4. Decaro N., Desario C., Parisi A., Martella V., Lorusso A., Miccolupo A., Mari V., Colaianni M.L., Cavalli A., Di Trani L., Buonavoglia C.: Genetic analysis of canine parvovirus type 2c. Virology 2009, 385, 5–10. 5. Dogonyaro B.B., Bosman A.M., Sibeko K.P., Venter E.H., van Vuuren M.: Genetic analysis of the VP2-encoding gene of canine parvovirus strains from Africa. Vet Microbiol 2013, 165, 460–465.
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|