Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
Author:
Klepp Laura I.12, Colombatti María A.12, Moyano Roberto D.12, Romano María I.12, Malovrh Tadej3, Ocepek Matjaž3, Blanco Federico C.12, Bigi Fabiana12
Affiliation:
1. Institute of Agrobiotechnology and Molecular Biology (IABIMO), INTA-CONICET Hurlingham , Argentina 2. Institute of Biotechnology, National Institute of Agricultural Technology , 1686 Hurlingham , Argentina 3. Institute for Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana , Ljubljana , Slovenia
Abstract
Abstract
Introduction
Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB.
Material and Methods
The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC).
Results
Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals.
Conclusion
The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.
Publisher
Walter de Gruyter GmbH
Subject
General Veterinary
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