Immunodetection of selected pancreatic hormones under intragastric administration of apelin-13, a novel endogenous ligand for an angiotensin-like orphan G-protein coupled receptor, in unweaned rats
Author:
Szymańczyk Sylwia1ORCID, Kras Katarzyna2ORCID, Osiak-Wicha Cezary2ORCID, Kapica Małgorzata1ORCID, Puzio Iwona1ORCID, Antushevich Hanna3ORCID, Kuwahara Atsukazu4ORCID, Kato Ikuo5ORCID, Arciszewski Marcin B.2ORCID
Affiliation:
1. Department of Animal Physiology , Lublin , Poland 2. Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin , Lublin , Poland 3. Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Department of Genetic Engineering , Jabłonna , Poland 4. Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka , Shizuoka , Japan 5. Department of Bioorganic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University , Kanazawa , Japan
Abstract
Abstract
Introduction
This study investigated the effects of intragastric administration of apelin-13 on the secretion of critical pancreatic hormones in a cohort of three-week-old Wistar rats. The research aimed to uncover apelin’s modulatory roles in endocrine interactions dictating metabolic homeostasis during early life.
Material and Methods
Rats were randomly assigned to control or experimental groups, receiving apelin-13 or saline for 14 days. The study population consisted of three-week-old Wistar rats of both sexes, weighing between 20 and 25 grams. Histological examination, analysis of variance and t-tests were employed to assess significant differences.
Results
Distinctive alterations in large islet morphology were observed, indicating a notable reduction in size. Additionally, an increase in alpha- and beta-cell density within specific islet sizes was noted, suggesting significant changes in cell populations. The study found a substantial increase in mitotic activity and a decrease in apoptosis in small and medium-sized islets post apelin-13 administration, indicating its potential role in regulating cell survival and proliferation.
Conclusion
The notable reduction in large islet size coupled with increased alpha and beta cell density implies a targeted impact of apelin-13 on pancreatic cell dynamics. Also, the observed increase in mitotic activity and decrease in apoptosis in small and medium-sized islets suggest its potential regulatory role in cell survival and proliferation within the pancreatic microenvironment.
Publisher
Walter de Gruyter GmbH
Reference34 articles.
1. Antushevich H., Bierla J., Pawlina B., Kapica M., Krawczynska A., Herman A.P., Kato I., Kuwahara A., Zabielski R.: Apelin’s effects on young rat gastrointestinal tract maturation. Peptides 2015, 65, 1–5, doi: 10.1016/j.peptides.2014.11.011. 2. Antushevich H., Pawlina B., Kapica M., Krawczynska A., Herman A.P., Kuwahara A., Kato I., Zabielski R.: Influence of fundectomy and intraperitoneal or intragastric administration of apelin on apoptosis, mitosis, and DNA repair enzyme OGG1,2 expression in adult rats gastrointestinal tract and pancreas. J Physiol Pharmacol 2013, 64, 423–428. 3. Antushevich H., Wójcik M.: Review: Apelin in disease. Clin Chim Acta 2018, 483, 241–248, doi: 10.1016/j.cca.2018.05.012. 4. Banaei-Bouchareb L., Gouon-Evans V., Samara-Boustani D., Castellotti M.C., Czernichow P., Pollard J.W., Polak M.: Insulin cell mass is altered in Csf1op/Csf1op macrophage-deficient mice. J Leukoc Biol 2004, 76, 359–367, doi: 10.1189/jlb.1103591. 5. Chaves-Almagro C., Auriau J., Dortignac A., Clerc P., Lulka H., Deleruyelle S., Projetti F., Nakhlé J., Frances A., Berta J., Gigoux V., Fourmy D., Dufresne M., Gomez-Brouchet A., Guillermet-Guibert J., Cordelier P., Knibiehler B., Jockers R., Valet P., Audigier Y., Masri B.: Upregulated Apelin Signaling in Pancreatic Cancer Activates Oncogenic Signaling Pathways to Promote Tumor Development. Int J Mol Sci 2022, 23, 10600, doi: 10.3390/ijms231810600.
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