Attenuative Role of Idebenone on Deltamethrin Mediated Peroxidative Toxicity of Ram Semen Stored at 4°C

Abstract

Abstract The current study was conducted to evaluate the different concentrations of deltamethrin (DEL; Exp. 1) and the protective role of idebenone (IDB) supplemented with toxic dose of deltamethrin (Exp. 2) during chilled storage of ram semen. Collected samples were pooled and diluted at 500×106 spermatozoa per mL. In Exp. 1, effect of DEL at 1, 2.5, 5, 10, 20, 40, 80 μM levels was evaluated on different variables of spermatozoa motion characteristics. In Exp. 2, different amounts of IDB (2, 4, and 8 μM) concurrent with constant doses of DEL (10 μM) were examined on semen quality upon chilled preservation up to 72 h. Indices of spermatozoa kinematics, functionality of plasma membrane and viability were recorded. Biochemical metabolites were measured in spermatozoa and its medium (extender) at different time points. In Exp. 1, different parameters of spermatozoa kinematics were affected by exposure to DEL in a dose dependent manner. In Exp. 2, combination of IDB with DEL resulted in a significant (P<0.05) increase in total motility, forward progressive motility and curvilinear path velocity compared to DEL group at 24, 48 and 72 h. Simultaneous administration of IDB with DEL increased the percentage of spermatozoa with functional membrane and viability compared to DEL group at 72 h (P<0.05). The amounts of lipid peroxidation index were lower in medium of combination groups compared to DEL group at 48 h and 72 h (P<0.05). Antioxidant capacity was higher in spermatozoa and medium of IDB treated groups compared to DEL group at 72 h (P<0.05). Amounts of total nitrate-nitrite and superoxide dismutase activity of spermatozoa and medium were not affected by treatment (P>0.05). In conclusion, IDB could ameliorate oxidative and peroxidative damages induced by DEL mild toxicity upon cold preservation of ram semen.