Author:
Novoselova E. G.,Glushkova O. V.,Khrenov M. O.,Lunin S. M.,Sharapov M. G.,Goncharov R. G.,Mubarakshina E. K.,Novoselova T. V.,Parfenyuk S. B.
Abstract
Abstract—The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the Tlr4 gene. Third, Tα1 significantly reduced p53 and the activity of the P53 gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in Ar-1 gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.
Subject
Structural Biology,Biophysics
Reference41 articles.
1. Gruver A.G., Ventevogel M.S., Sempowski G.D. 2009. Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia induced thymus involution. J. Endocrinol.
203, 75–85.
2. Haynes B.F., Markert M.L., Sempowski G.D., Patel D.D., Hale L.P. 2000. The role of the thymus in immune reconstitution in aging, bone marrow transplantation, and HIV-1 infection. Annu. Rev. Immunol.
18, 529–560.
3. Franceschi C., Bonafè M., Valensin S., Olivieri F., De Luca M., Ottaviani E., De Benedictis G. 2000. Inflamm-aging. An evolutionary perspective on immunosenescence. Ann. N.Y. Acad. Sci. 908, 44–54.
4. Lunin S.M., Khrenov M.O., Novoselova T.V., Parfenyuk S.B., Novoselova E.G. 2008. Thymulin, a thymic peptide, prevents the overproduction of pro-inflammatory cytokines and heat shock protein hsp70 in inflammation-bearing mice. Immunol. Invest.
37, 858–870.
5. Lunin S.M., Novoselova T.V., Khrenov M.O., Glushkova O.V., Parfenyuk S.B., Smolikhina T.I., Fesenko E.E., Novoselova E.G. 2009. Immunomodulatory effects of thymopentin under acute and chronic inflammations in mice. Biopysics (Moscow). 54. 179–181.