Photochemical Properties and Reactions with Biomolecules of 4'-N-Acetyl Derivative of Norfloxacin

Author:

Zhang Peng1,Li Haixia1,Liu Yancheng1,Yao Side1,Wang Wenfeng

Affiliation:

1. Chinese Academy of Sciences, Shanghai Institute of Applied Physics, Shanghai 201800, Volksrepublik China

Abstract

Abstract The photochemical properties of 4'-N-acetyl derivative of norfloxacin (ANFX) were investigated in different solutions. Both UV-Vis absorption and the quantum yields of excited states are pH-dependent, and pK a value of ground state for the protonation of 3-carboxylic group was determined to be 6.5 ± 0.2. Pulse radiolysis and laser flash photolysis experiments were carried out to characterize transient species of ANFX and to investigate reactions with tryptophan (TrpH) and 2'-seoxyguanosine-5'-monophosphoric acid disodium salt (dGMP). The ANFX undergoes the photoejection of electron by a mixed mechanism of one-photon and two-photon processes. Under moderate laser energy conditions, two-photon process is predominantly. The ANFX radical dianion (ANFX(–H)·2−) formed in reaction with eaq is characterized by the absorption around 370 nm, and the rate constant was determined to be 1.2 × 1010 dm3 mol−1 s−1. The 3ANFX(–H)−∗ is able to oxidize TrpH and dGMP with bimolecular rate constants of 5.4 × 108 and 9.5 × 106 dm3 mol−1 s−1, respectively. The ANFX(–H)·2− and the oxidized radicals of TrpH and dGMP were observed directly. Under aerobic conditions, the photo-oxidations involve both type I and type II mechanisms.

Publisher

Walter de Gruyter GmbH

Subject

Physical and Theoretical Chemistry

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