Alive Animal Model for Epilepsy by Intradorsal Striatum Injection of Colchicine

Author:

Fakhroleslam Zahra1,Karami Manizheh1,Roghani Mehrdad2,Nejadebrahim Atiyeh1

Affiliation:

1. Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

2. Department of Physiology, Faculty of Medicine, Shahed University, Tehran, Iran

Abstract

Abstract Background Epilepsy is a neural disorder with repeatable seizure attacks. In this article, we used the neurotoxin colchicine, which is derived from the plant Colchicum autumnale, to introduce a low cost but the more valuable alive animal model for epilepsy. Materials and Methods Wistar rats weighing 250 to 300 g after intraperitoneal injection of ketamine (100 mg/kg) and xylazine (20 mg/kg) were restrained in the stereotaxic apparatus; they were cannulated in the dorsal striatal area (AP: 0.5 mm; L: 3 mm; V: 3.6 mm). One week later, an injection cannula attached to a 5-µ Hamilton syringe by polyethylene tubing guided 0.05 to 25 μg/rat colchicine in the recovered healthy rats once daily for 4 consecutive days. The control group solely received the saline solution. The behavioral signs of all animals were daily recorded. Finally, the brains of rats under deep euthanasia were collected in 10% formalin and examined histopathologically. The dorsal striatal regions were cut coronally into 3 to 4 µm-thick slices, and then stained with hematoxylin-eosin. They were eventually examined under the light microscope to verify the injection placement or possibility of lesions. All data were analyzed by analysis of variance under α = 0.05. Results Behaviors were quantified based on Racine five-stage scoring and showed the significant epileptic generalized seizures in alive animal treated by intrastriatal injection of colchicine. However, tissue damage was invisible in the target brain area. Conclusion The colchicine, using injection successively into the dorsal striatal region of rat, can create recurring epileptic convulsions in the animal.

Publisher

Georg Thieme Verlag KG

Subject

Clinical Neurology

Reference34 articles.

1. Animal models of epilepsy: use and limitations;L Kandratavicius;Neuropsychiatr Dis Treat,2014

2. Animal models in epilepsy research: legacies and new directions;B P Grone;Nat Neurosci,2015

3. Mesial temporal lobe epilepsy: pathogenesis, induced rodent models and lesions;A K Sharma;Toxicol Pathol,2007

4. Animal models of epileptogenesis;H S White;Neurology,2002

5. Use of colchicine in cortical area 1 of the hippocampus impairs transmission of non-motivational information by the pyramidal cells;N R Zaniani;Basic Clin Neurosci,2013

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3