Pharmacokinetics, Tissue Distribution, and Excretion Study of Cajanonic Acid A in Rats by UPLC-MS/MS
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Published:2020-02-11
Issue:05
Volume:86
Page:312-318
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ISSN:0032-0943
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Container-title:Planta Medica
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language:en
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Short-container-title:Planta Med
Author:
Zhang Li1,
Chen Rui2,
Ban Yujuan1,
Cai Jin1,
Peng Jingang1,
Huang Jing1,
Wang Jianta12,
Chen Wenzhang2,
Gao Xiuli1,
Zhou Xunrong3,
Tang Lei12
Affiliation:
1. State Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmacy, Guizhou Medical University, Guiyang, China
2. Guizhou Provincial Engineering Technology Research Center for Chemical Drug R&D, Guiyang, China
3. The Second Affiliated Hospital of Guizhou Chinese Traditional Medicine University, Guiyang, China
Abstract
AbstractCajanonic acid A (CAA), a prenylated stilbene derivative extracted from the leaves of pigeon pea (Cajanus cajan), has been reported to possess inhibitory activity on the peroxisome proliferator-activated receptor gamma (PPARγ) and protein tyrosine phosphatase 1B (PTP1B). Its hypoglycemic activity in rats is comparable to that of the approved antidiabetic agent rosiglitazone. Therefore, CAA is a potential candidate for the treatment of type 2 diabetes and a lead compound for the discovery of novel hypoglycemic drugs. To achieve a thorough understanding of the biological behavior of CAA in vivo, our current study was designed to investigate the pharmacokinetics, bioavailability, distribution, and excretion of CAA in rats by UPLC-MS/MS. Chromatographic separation was performed on BEHC18 column (2.1 mm × 50 mm, 1.7 µm). Quantification was performed under the negative ion mode by using single reaction monitoring (SRM) of the transitions of m/z 353.14 → 309.11 for CAA and m/z 269.86 → 224.11 for genistein, respectively. Standard calibration curve showed excellent linearity (r2 > 0.99) within the range of 2 – 800 ng/mL. The accuracies and precisions were within the acceptance limits (all < 20%). CAA was quickly absorbed into bloodstream and distributed rapidly and widely to various tissues. The excretion ratio of CAA in the 3 main pathways via bile, feces, and urine was only 5.17%. These results indicate that CAA was quickly and thoroughly metabolized in vivo and excreted mainly as metabolites.
Funder
Guizhou innovative talent foundation
first-class discipline construction project in Guizhou Province-Public Health and Preventive Medicine
the Science Fundation of Guiyang
Foundation of Guizhou Educational Committee
National natural science foundation of China
The Social Development of Technical Supporting Foundation of Guizhou Province
Publisher
Georg Thieme Verlag KG
Subject
Organic Chemistry,Complementary and alternative medicine,Drug Discovery,Pharmaceutical Science,Pharmacology,Molecular Medicine,Analytical Chemistry
Cited by
2 articles.
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