Suffrutines A and B Inhibit the Expression of Inflammatory Mediators in LPS-Induced RAW264.7 Cells by Suppressing the NF-κB Signaling Pathway

Author:

Chen Chun1,Zhu Ze-Feng12,Nie Wen-Xing1,Zou Yong13

Affiliation:

1. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China

2. Department of Pharmacy, the Fifth Affiliated Hospital of Jinan University, Heyuan, Guangdong, China

3. Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Guangzhou, Guangdong, China

Abstract

Abstract Flueggea suffruticosa is a traditional Chinese medicine that has been commonly used for the treatment of inflammatory ailments, including rheumatism and lumbago. Suffrutines A and suffrutines B are a pair of novel E,E and Z,E isomeric indolizidine alkaloids isolated from the roots of F. suffruticosa. However, their anti-inflammatory activity has not been reported thus far. The aim of this study was to investigate the inhibitory effect of inflammatory mediators and possible mechanisms of suffrutines A and B in lipopolysaccharide-induced RAW264.7 cells. Results showed that suffrutines A and B could remarkably inhibit the production of nitric oxide, prostaglandin E2, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2 in lipopolysaccharide-induced RAW264.7 cells. Further evaluation demonstrated that compared with suffrutines A, suffrutines B could more significantly inhibit the phosphorylation of IKKα/β, the degradation of IκBα, and the nuclear translocation of the p65 and p52 subunits in the canonical and non-canonical nuclear factor-κB pathways. Therefore, suffrutines B exhibited more potent inhibitory activity on inflammatory mediators than suffrutines A.

Funder

The National Natural Science Foundation of China

The National Key Research and Development Project of China

The Key Project of Guangdong Natural Science Foundation

STPGC

Basic and Applied Basic Research Foundation of Guangdong Province

Guangdong Provincial Key Laboratory of Construction Foundation

Publisher

Georg Thieme Verlag KG

Subject

Organic Chemistry,Complementary and alternative medicine,Drug Discovery,Pharmaceutical Science,Pharmacology,Molecular Medicine,Analytical Chemistry

Reference33 articles.

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