Development of an efficient and scalable bioprocess for the plant hormone 12-OPDA: Overcoming the hurdles of nature's biosynthesis-Reference-Cited by-同舟云学术

Development of an efficient and scalable bioprocess for the plant hormone 12-OPDA: Overcoming the hurdles of nature's biosynthesis

Published:2024 Issue:26 Volume:22 Page:5406-5413
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Guntelmann Tim Lukas¹^ORCID,Dietz Karl-Josef²^ORCID,Gröger Harald¹^ORCID

Affiliation:

1. Chair of Industrial Organic Chemistry and Biotechnology, Faculty of Chemistry, Bielefeld University, Universitätsstr. 25, 33615, Bielefeld, Germany

2. Chair of Plant Biochemistry and Physiology, Faculty of Biology, Bielefeld University, Universitätsstr. 25, 33615, Bielefeld, Germany

Abstract

A biocatalytic cascade synthesis of cis-(+)-12-OPDA at 5–20 gL−1 substrate loading was developed, leading to excellent conversion, chemical purity (>99%), enantio- and diastereomeric excess (>99% ee, 96% de) as well as neglectable side-product (<1%).

Funder

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/OB/D4OB00258J

Reference28 articles.

1. Structural insights into the evolutionary paths of oxylipin biosynthetic enzymes

2. A plant oxylipin, 12-oxo-phytodienoic acid, inhibits proliferation of human breast cancer cells by targeting cyclin D1

3. 12-oxo-phytodienoic acid, a plant-derived oxylipin, attenuates lipopolysaccharide-induced inflammation in microglia

4. Cytoprotective effects of 12-oxo phytodienoic acid, a plant-derived oxylipin jasmonate, on oxidative stress-induced toxicity in human neuroblastoma SH-SY5Y cells

5. OPDAylation of Thiols of the Redox Regulatory Network In Vitro