Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection-Reference-Cited by-全球学者库

Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection

Published:2021 Issue:7 Volume:146 Page:2126-2130
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Chen Xue¹²³⁴⁵,Cheng Ying¹²³⁴⁵,Wang Yafen⁶⁷⁸⁹⁵,Tang Jing¹²³⁴⁵,Wang Fang¹²³⁴⁵,Chen Zilin¹²³⁴⁵^ORCID

Affiliation:

1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery

2. Ministry of Education

3. Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals

4. and Wuhan University School of Pharmaceutical Sciences

5. Wuhan

6. College of Chemistry and Molecular Sciences

7. Key Laboratory of Biochemical Polymers of Ministry of Education. The Institute for Advanced Studies

8. Hubei Province Key Laboratory of Allergy and Immunology

9. Wuhan University

Abstract

A simple and label-free fluorescence method is developed for the highly sensitive detection of TET1 based on ThT/G-quadruplexes in combination with the specific design of oligonucleotides.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00109D

Reference35 articles.

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