Harnessing the catalytic plasticity of the ent-kaurene synthase from Bradyrhizobium japonicum to produce the ent-rosane and ent-pimarane scaffolds-Reference-Cited by-同舟云学术

Harnessing the catalytic plasticity of the ent-kaurene synthase from Bradyrhizobium japonicum to produce the ent-rosane and ent-pimarane scaffolds

Published:2024 Issue:2 Volume:14 Page:306-314
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Zhang Fan¹²,Wang Su-Jing³,Xiao Wen³,Yu Ming-Zhu³,Sha Feng³,Wu Ruibo¹^ORCID,Xiang Zheng³⁴^ORCID

Affiliation:

1. Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China

2. Guangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, P. R. China

3. State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Genomics, AI for Science (AI4S) Preferred Program, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China

4. Shenzhen Bay Laboratory, Gaoke Innovation Center, Institute of Chemical Biology, Guangqiao Road, Guangming District, Shenzhen 518132, China

Abstract

The F72Y mutation of the ent-kaurene synthase from Bradyrhizobium japonicum leads to the formation of ent-rosa-5(10),15-diene and ent-pimara-8,15-diene, two common scaffolds of labdane diterpenoids.

Funder

National Natural Science Foundation of China

Science and Technology Foundation of Shenzhen City

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CY/D3CY00115F

Reference49 articles.

1. Structural and Chemical Biology of Terpenoid Cyclases

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3. A Single Residue Switch Converts Abietadiene Synthase into a Pimaradiene Specific Cyclase

4. Following evolution's lead to a single residue switch for diterpene synthase product outcome

5. Increasing Complexity of a Diterpene Synthase Reaction with a Single Residue Switch