Nanoscale probing and imaging of HIV-1 RNA in cells with a chimeric LNA–DNA sensor-Reference-Cited by-同舟云学术

Nanoscale probing and imaging of HIV-1 RNA in cells with a chimeric LNA–DNA sensor

Published:2022 Issue:8 Volume:14 Page:3049-3061
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Amodio Alessia¹²^ORCID,Cassani Marco¹³^ORCID,Mummolo Liviana¹,Cortez-Jugo Christina¹^ORCID,Bhangu Sukhvir Kaur⁴^ORCID,Symons Jori⁵,Ahlenstiel Chantelle L.⁶,Forte Giancarlo³^ORCID,Ricci Francesco²^ORCID,Kelleher Anthony D.⁶^ORCID,Lewin Sharon R.⁵⁷⁸,Cavalieri Francesca²⁴^ORCID,Caruso Frank¹^ORCID

Affiliation:

1. Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia

2. Dipartimento di Scienze e Tecnologie Chimiche, Università degli Studi di Roma Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy

3. International Clinical Research Center (ICRC), St Anne's University Hospital, CZ-65691 Brno, Czech Republic

4. School of Science, RMIT University, Victoria 3000, Australia

5. Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia

6. Kirby Institute, University of New South Wales, New South Wales 2052, Australia

7. Victorian Infectious Diseases Service, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia

8. Department of Infectious Diseases, Alfred Hospital and Monash University, Melbourne, Victoria 3004, Australia

Abstract

A chimeric locked nucleic acid (LNA)–DNA sensor enables hybridization chain reaction (HCR) for the efficient detection and nanoscale imaging of HIV-1 RNA transcripts in cell lysates, and fixed and live cells.

Funder

Royal Melbourne Institute of Technology

European Regional Development Fund

National Health and Medical Research Council

European Commission

Australian Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2022/NR/D1NR08418F

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