Two stage, nested isothermal amplification in a single tube

Author:

El-Tholoth Mohamed12345ORCID,Anis Eman678910,Bau Haim H.1191213

Affiliation:

1. Department of Virology

2. Faculty of Veterinary Medicine

3. Mansoura University

4. Mansoura 35516

5. Egypt

6. Department of Pathobiology

7. New Bolton Center

8. School of Veterinary Medicine

9. University of Pennsylvania

10. Kennett Square

11. Department of Mechanical Engineering and Applied Mechanics

12. Philadelphia

13. USA

Abstract

Sensitive, specific and rapid molecular diagnosis of respiratory diseases in animals and humans is critical to facilitate appropriate control measures and treatment.

Funder

Foundation for the National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference40 articles.

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2. M. García , S. J.Spatz and J. S.Guy , in Diseases of Poultry , ed. D. E. Swayne , J. R. Glisson , L. R. McDougald , L. K. Nolan , D. L. Suarez and V. Nair , Blackwell Publishing , Ames, Iowa , 2013 , 13th edn, pp. 161–179

3. Molecular epidemiology of infectious laryngotracheitis: a review

4. Genomic sequence analysis of the United States infectious laryngotracheitis vaccine strains chicken embryo origin (CEO) and tissue culture origin (TCO)

5. Coronaviruses in poultry and other birds

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