Genetic analysis with pyrosequencing using loop pipetting and a light dependent resistor-Reference-Cited by-同舟云学术

Genetic analysis with pyrosequencing using loop pipetting and a light dependent resistor

Published:2021 Issue:42 Volume:13 Page:5035-5047
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Iqbal Faisal¹,Shabbir Muhammad Imran¹^ORCID

Affiliation:

1. Department of Biological Sciences, Faculty of Basic & Applied Sciences, International Islamic University, Sector H-10, Islamabad, Pakistan

Abstract

A DNA sequencer called LoopSeeq was developed using pyrosequencing chemistry where iterative addition of dNTPs was performed by contact dispensing through a loop pipette and detection of chemiluminescence with inexpensive light sensor LDR for point-of-care applications.

Funder

Higher Education Commission, Pakistan

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D1AY01123E

Reference32 articles.

1. A decade’s perspective on DNA sequencing technology

2. K. K.Jerzy , Next Generation Sequencing - Advances, Applications and Challenges , In Tech Publishers , 2016 , pp. 3–60 , https://www.intechopen.com/chapters/49602

3. Method Enabling Pyrosequencing on Double-Stranded DNA

4. Pyrosequencing Sheds Light on DNA Sequencing

5. A Sequencing Method Based on Real-Time Pyrophosphate

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