Versatile, facile and low-cost single-cell isolation, culture and sequencing by optical tweezer-assisted pool-screening-Reference-Cited by-同舟云学术

Versatile, facile and low-cost single-cell isolation, culture and sequencing by optical tweezer-assisted pool-screening

Published:2023 Issue:1 Volume:23 Page:125-135
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Xu Teng¹²³^ORCID,Li Yuandong¹²³,Han Xiao¹⁴,Kan Lingyan¹²³,Ren Jing¹²³,Sun Luyang¹²³,Diao Zhidian¹²³,Ji Yuetong¹⁵,Zhu Pengfei¹⁵,Xu Jian¹²³⁶,Ma Bo¹²³⁶

Affiliation:

1. Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China

2. Shandong Energy Institute, Qingdao, China

3. Qingdao New Energy Shandong Laboratory, Qingdao, China

4. School of Chemistry and Chemical Engineering, University of Jinan, Jinan, China

5. Single-Cell Biotechnology Ltd., Qingdao, Shandong, China

6. Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

Abstract

An optical tweezer-assisted pool-screening and single-cell isolation system is established for precise, indexed isolation of individual cells, with features of versatility, facileness, flexibility, modularized design, and low cost.

Funder

Natural Science Foundation of Shandong Province

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2023/LC/D2LC00888B

Reference50 articles.

1. Dissecting genomic diversity, one cell at a time