A single-step high-throughput bioassay for quantifying Fc-containing recombinant proteins based on non-classical calculation of fluorescence polarization-Reference-Cited by-同舟云学术

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A single-step high-throughput bioassay for quantifying Fc-containing recombinant proteins based on non-classical calculation of fluorescence polarization

Published:2024 Issue:24 Volume:16 Page:3917-3926
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Zheng Yujuan¹²^ORCID,Chen Ganjun³,Liu Guojian⁴,Rana Gul E.¹²^ORCID,Wang Chunhe¹³²^ORCID

Affiliation:

1. Biotherapeutics Discovery Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

2. University of Chinese Academy of Sciences, Beijing 100049, China

3. Dartsbio Pharmaceuticals Co., Ltd, Zhongshan 528400, China

4. Shanghai Mabstone Biotechnology Co., Ltd, Shanghai 201203, China

Abstract

A single-step and high-throughput bioassay based on non-classical calculation of fluorescence polarization for quantifying Fc-containing recombinant proteins in the cell culture supernatant.

Funder

Special Project for Research and Development in Key Areas of Guangdong Province

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AY/D4AY00372A

Reference27 articles.

1. Biopharmaceutical benchmarks 2022

2. Biopharmaceutical Manufacturing: Historical Perspectives and Future Directions

3. Biopharmaceutical benchmarks 2018

4. Pharmacokinetics of monoclonal antibodies and Fc-fusion proteins

5. Fc fusion as a platform technology: potential for modulating immunogenicity

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