Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

Author:

Li Huimin12ORCID,Xie Yi12ORCID,Chen Fumin12ORCID,Bai Huiwen3ORCID,Xiu Leshan12ORCID,Zhou Xiaonong12ORCID,Guo Xiaokui12ORCID,Hu Qinqin12ORCID,Yin Kun12ORCID

Affiliation:

1. School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China

2. One Health Center, Shanghai Jiao Tong University-The University of Edinburgh, Shanghai, People's Republic of China

3. Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, 220 South 33rd St., Philadelphia, Pennsylvania, USA

Abstract

This review summarizes the recent advances and the main strategies to improve the sensitivity of amplification-free CRISPR/Cas-based detection techniques.

Funder

Natural Science Foundation of Shanghai

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

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