A rapid nucleic acid concentration measurement system with large field of view for a droplet digital PCR microfluidic chip-Reference-Cited by-同舟云学术

A rapid nucleic acid concentration measurement system with large field of view for a droplet digital PCR microfluidic chip

Published:2021 Issue:19 Volume:21 Page:3742-3747
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Shen Jinrong¹^ORCID,Zheng Jihong¹,Li Zhenqing¹^ORCID,Liu Yourong¹,Jing Fengxiang²,Wan Xinjun¹,Yamaguchi Yoshinori³,Zhuang Songlin¹

Affiliation:

1. Shanghai Key Lab of Modern Optical System, School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

2. Shanghai Turtle Technology Limited, Shanghai 200439, China

3. Oono Joint Research laboratory, Graduate School of Engineering, Osaka University, Osaka, Japan

Abstract

Droplet digital polymerase chain reaction (ddPCR) is an effective technique, with unparalleled sensitivity, for the absolute quantification of target nucleic acids.

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/LC/D1LC00532D

Reference27 articles.

1. Absolute quantification and analysis of extracellular vesicle lncRNAs from the peripheral blood of patients with lung cancer based on multi-colour fluorescence chip-based digital PCR

2. Absolute quantification of Vibrio parahaemolyticus by multiplex droplet digital PCR for simultaneous detection of tlh, tdh and ureR based on single intact cell

3. A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System

4. The development of a qPCR assay to detect tick (Ixodida) DNA and its implementation for the study of tick-borne pathogen transmission

5. Development and validation of a qPCR-based method for quantifying Shiga toxin-encoding and other lambdoid bacteriophages

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