High-throughput sperm DNA analysis at the single-cell and population levels-Reference-Cited by-同舟云学术

High-throughput sperm DNA analysis at the single-cell and population levels

Published:2023 Issue:16 Volume:148 Page:3748-3757
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Simchi Mohammad¹^ORCID,Riordon Jason¹^ORCID,Wang Yihe¹,McCallum Christopher¹,You Jae Bem¹^ORCID,Jarvi Keith²,Nosrati Reza³^ORCID,Sinton David¹^ORCID

Affiliation:

1. Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario M5S 3G8, Canada

2. Department of Surgery, Division of Urology, Mount Sinai Hospital, University of Toronto, 60 Murray Street, 6th Floor, Toronto, Ontario M5T 3L9, Canada

3. Department of Mechanical and Aerospace Engineering, Monash University, 20 Research Way, Clayton Campus, VIC 3800, Australia

Abstract

An automated analysis method for the sperm chromatin dispersion assay is demonstrated that quantifies DNA quality of more than 1000 of sperm at the single-cell and population levels within 7 minutes – a 40-fold reduction in processing time.

Funder

Canadian Institutes of Health Research

Natural Sciences and Engineering Research Council of Canada

Australian Research Council

Canada Research Chairs

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D3AN00564J

Reference53 articles.

1. WHO infertility fact sheet, https://www.who.int/news-room/fact-sheets/detail/infertility

2. A unique view on male infertility around the globe

3. Microfluidics for sperm analysis and selection

4. World Health Organization , WHO laboratory manual for the examination and processing of human semen , 6th edn, 2021

5. Longitudinal semen parameter assessments and live birth: variability and implications for treatment strategies

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