Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers-Reference-Cited by-同舟云学术

Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers

Published:2022 Issue:47 Volume:13 Page:14032-14040
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Tang Heng¹,Peng Junran¹,Peng Shuang¹,Wang Qi¹,Jiang Xin¹,Xue Xiaocheng¹,Tao Yanxin²,Xiang Limin¹,Ji Quanjiang³^ORCID,Liu Song-Mei²,Weng Xiaocheng¹^ORCID,Zhou Xiang¹^ORCID

Affiliation:

1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, 430072, Hubei, P. R. China

2. Department of Clinical Laboratory, Center for Gene Diagnosis, and Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 430072, Hubei, P. R. China

3. School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, P. R. China

Abstract

The development of RNA imaging strategies in live cells is essential to improve our understanding of their role in various cellular functions.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/SC/D2SC04656C

Reference42 articles.