Detection of cannabinoid receptor type 2 in native cells and zebrafish with a highly potent, cell-permeable fluorescent probe

Author:

Gazzi Thais1,Brennecke Benjamin1,Atz Kenneth2,Korn Claudia2,Sykes David34,Forn-Cuni Gabriel5,Pfaff Patrick6,Sarott Roman C.6ORCID,Westphal Matthias V.6,Mostinski Yelena1,Mach Leonard1,Wasinska-Kalwa Malgorzata1,Weise Marie1,Hoare Bradley L.34,Miljuš Tamara34,Mexi Maira34,Roth Nicolas7,Koers Eline J.34,Guba Wolfgang2,Alker André2,Rufer Arne C.2,Kusznir Eric A.2,Huber Sylwia2,Raposo Catarina2,Zirwes Elisabeth A.2,Osterwald Anja2,Pavlovic Anto2,Moes Svenja2,Beck Jennifer2,Nettekoven Matthias2,Benito-Cuesta Irene8,Grande Teresa8,Drawnel Faye2,Widmer Gabriella2,Holzer Daniela2,van der Wel Tom9,Mandhair Harpreet10ORCID,Honer Michael2,Fingerle Jürgen2,Scheffel Jörg1112,Broichhagen Johannes1ORCID,Gawrisch Klaus13,Romero Julián8,Hillard Cecilia J.14,Varga Zoltan V.1315,van der Stelt Mario9,Pacher Pal13,Gertsch Jürg10,Ullmer Christoph2,McCormick Peter J.7,Oddi Sergio1617,Spaink Herman P.5ORCID,Maccarrone Mauro1718,Veprintsev Dmitry B.34,Carreira Erick M.6,Grether Uwe2,Nazaré Marc1ORCID

Affiliation:

1. Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Campus Berlin-Buch, 13125 Berlin, Germany

2. Roche Pharma Research & Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., 4070 Basel, Switzerland

3. Faculty of Medicine & Health Sciences, University of Nottingham, Nottingham NG7 2UH, England, UK

4. United Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, England, UK

5. Leiden University, Einsteinweg 55, 2333 CC Leiden, the Netherlands

6. Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule Zürich, Vladimir-Prelog-Weg 3, 8093 Zürich, Switzerland

7. William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University of London, London EC1M 6BQ, England, UK

8. Faculty of Experimental Sciences, Universidad Francisco de Vitoria, Pozuelo de Alarcón, 28223, Madrid, Spain

9. Department of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, 2333 CC, Leiden, the Netherlands

10. Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland

11. Dermatological Allergology, Allergie-Centrum-Charité, Department of Dermatology and Allergy, Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany

12. Allergology, Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Berlin, Germany

13. National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD 20852, USA

14. Department of Pharmacology and Toxicology, Neuroscience Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA

15. HCEMM-SU Cardiometabolic Immunology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, 1085 Budapest, Hungary

16. Faculty of Veterinary Medicine, University of Teramo, 64100 Teramo, European, Italy

17. European Center for Brain Research (CERC), Santa Lucia Foundation, 00179 Rome, Italy

18. Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy

Abstract

Detection and visualization of the cannabinoid receptor type 2 by a cell-permeable high affinity fluorescent probe platform enables tracing receptor trafficking in live cells and in zebrafish.

Funder

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

Ministero dell'Istruzione, dell'Università e della Ricerca

National Institute on Alcohol Abuse and Alcoholism

Eidgenössische Technische Hochschule Zürich

Scientific Systems Company, Incorporated

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

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