Author:
Tan Yusheng,Wang Fenghua,Chen Xia,Wang Jinshan,Zhao Qi,Li Shuang,Wang Zefang,Fu Sheng,Chen Cheng,Yang Haitao
Abstract
Porcine epidemic diarrhea virus(PEDV) mainly infects neonatal pigs, resulting in significant morbidity and mortality. Owing to problems such as long periods of virus shedding, existing vaccines cannot provide complete protection from PEDV infection. The PEDV genome encodes two polyprotein precursors required for genome replication and transcription. Each polyprotein undergoes extensive proteolytic processing, resulting in functional subunits. This process is mainly mediated by its genome-encoded main protease, which is an attractive target for antiviral drug design. In this study, the main protease ofPorcine epidemic diarrhea virusin complex with a Michael acceptor was crystallized. The complex crystals diffracted to 2.5 Å resolution and belonged to space groupR3, with unit-cell parametersa= 175.3,b= 175.3,c= 58.7 Å. Two molecules were identified per asymmetric unit.
Publisher
International Union of Crystallography (IUCr)
Subject
Condensed Matter Physics,Genetics,Biochemistry,Structural Biology,Biophysics
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