Clonal dynamics of haematopoiesis across the human lifespan

Author:

Mitchell Emily,Spencer Chapman MichaelORCID,Williams NicholasORCID,Dawson Kevin J.,Mende NicoleORCID,Calderbank Emily F.ORCID,Jung Hyunchul,Mitchell Thomas,Coorens Tim H. H.ORCID,Spencer David H.,Machado Heather,Lee-Six HenryORCID,Davies Megan,Hayler Daniel,Fabre Margarete A.,Mahbubani KrishnaaORCID,Abascal FedericoORCID,Cagan AlexORCID,Vassiliou George S.ORCID,Baxter Joanna,Martincorena InigoORCID,Stratton Michael R.ORCID,Kent David G.ORCID,Chatterjee KrishnaORCID,Parsy Kourosh SaebORCID,Green Anthony R.,Nangalia JyotiORCID,Laurenti ElisaORCID,Campbell Peter J.ORCID

Abstract

AbstractAge-related change in human haematopoiesis causes reduced regenerative capacity1, cytopenias2, immune dysfunction3 and increased risk of blood cancer4–6, but the reason for such abrupt functional decline after 70 years of age remains unclear. Here we sequenced 3,579 genomes from single cell-derived colonies of haematopoietic cells across 10 human subjects from 0 to 81 years of age. Haematopoietic stem cells or multipotent progenitors (HSC/MPPs) accumulated a mean of 17 mutations per year after birth and lost 30 base pairs per year of telomere length. Haematopoiesis in adults less than 65 years of age was massively polyclonal, with high clonal diversity and a stable population of 20,000–200,000 HSC/MPPs contributing evenly to blood production. By contrast, haematopoiesis in individuals aged over 75 showed profoundly decreased clonal diversity. In each of the older subjects, 30–60% of haematopoiesis was accounted for by 12–18 independent clones, each contributing 1–34% of blood production. Most clones had begun their expansion before the subject was 40 years old, but only 22% had known driver mutations. Genome-wide selection analysis estimated that between 1 in 34 and 1 in 12 non-synonymous mutations were drivers, accruing at constant rates throughout life, affecting more genes than identified in blood cancers. Loss of the Y chromosome conferred selective benefits in males. Simulations of haematopoiesis, with constant stem cell population size and constant acquisition of driver mutations conferring moderate fitness benefits, entirely explained the abrupt change in clonal structure in the elderly. Rapidly decreasing clonal diversity is a universal feature of haematopoiesis in aged humans, underpinned by pervasive positive selection acting on many more genes than currently identified.

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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