Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology
Link
http://www.nature.com/articles/nprot.2017.145.pdf
Reference48 articles.
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2. Weeks, D.P., Spalding, M.H. & Yang, B. Use of designer nucleases for targeted gene and genome editing in plants. Plant Biotechnol. J. 14, 483–495 (2016).
3. Voytas, D.F. Plant genome engineering with sequence-specific nucleases. Annu. Rev. Plant Biol. 64, 327–350 (2013).
4. Chen, K. & Gao, C. Targeted genome modification technologies and their applications in crop improvements. Plant Cell Rep. 33, 575–583 (2014).
5. Hsu, P.D., Lander, E.S. & Zhang, F. Development and applications of CRISPR-Cas9 for genome engineering. Cell 157, 1262–1278 (2014).
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